I had a PS2 game in my comp I tried UltraISO and Alcohol 120% with many ways but my PS2 still doesnt work games :/

I don't get it...you want to make an image (ISO) of the game or you tried to make it but the game is not working?
Or you're trying to burn the game into dvd and play it on your PS2(not pcsx2)

Yeah, I changed it to PS2 format from ISO , I want to play it on my PS2(not pcsx2)

Well do you have any way to boot backups(modchip,swap magic,esr,memor32....)