What game is it?
Followed the configuration guide? Any other error before that errors?
What happens if you try running the game from the Disc using Gigaher'z CDVD plugin or P.E.Op.S CDVD plugin?
What CDVD plugin are you using right now? have you mounted the image somewhere else?
i'm using Gigaher'z CDVD plugin and mounting from deamon tools
Well dont do that, unmount it and run it with Linuz ISO cdvd plugin.
Nop not working... same error
Go in the GSdx configuration and in Interlacing select None. That might fix this